1
Synthesis of plant
Growth
Promoter
(Natural
Liquid Fertilizers):

Leaves
of various medicinal
plant e.g. Ricinus
communis (Caster),
Annona
squamosa (Custard
apple),
Tinospora
cordifolia (Gulvel),
Azadirachta
indica (Neem
Tree),
Nicotiana tobacum (Tobacco)
taken in equal proportion (250gm)
and fermented for one month in cow urine (Gomutra).
Filtered it and filtrate was taken as end product.

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2
Effect
of plant
Growth
Promoter
(Natural
Liquid Fertilizers)on
seed germination:

phytotoxicity
can assess by a seed germination assay, it is one of the most common
techniques (Kapanen and Itavaara, 2001). The graded concentrations
(5,10,30 and 50 µl/ml)
of fermented
plant extracts (FPEs) were added
aseptically to sterilized petriplateslined with Whatman no. 1 filter
paper. Surface sterilized seeds of Mung weregerminated (20 seed per
plate) in each concentration of PEFs. Similar experiment without
PEFwas conducted as control. Distilled water was used as a control
set for the testing of seed germination (Hoekstra et al. 2002;
Fuentes et al. 2004). After 10
days of treatment, seedlings were harvested and shoot and roots of
seedling were separated.Seedling growth in terms of root length,shoot
length, fresh weight and dry weightwere recorded and results were
compared to see effect of PEFs on seed germination and early seedling
growth.

RESULTS
AND DISCUSSION
Results
pertaining to seed germination and
early seedling growth clearly indicate that PEFs at lower
concentration promoted seed germination and seedling growth, but at
higher concentration reduced seed germination and seedling growth.
Lower concentrations of PEFS (5,10
µl/ml)
showed significant enhancement in shoot and root lengths, however
higher concentrations (50
µl/ml)
of PEFsshowed decreased root length, shoot length and total seedlings
height. There was no major difference in root shoot ratio in all
treatments however an decreasing trend was seen from lower to higher
concentrations. Similar trend as in seedling height was seen in case
of fresh and dry weight of the seedlings after PEFs treatments
(Table. 1).

Table
No.1 Effect of Fermented product on seedling growth in Mung

Concentration

(µl/ml)

(µl/

Shoot
Length (cm)

Root
length(cm)

Total
seedlings Height (cm)

Root-Shoot
Ratio

Fresh
Wt. (mg)

Dry Wt. (mg)

00

3.05

1.76

4.81

0.58

529.12

73.38

05

4.86

2.48

7.36

0.51

676.28

90.44

10

6.45

2.88

9.33

0.45

768.92

112.38

30

5.85

2.40

8.25

0.41

698.08

101.12

50

230

0.93

2.66

0.40

356.26

41.42

Fruits and vegetables are a rich source of B (Bellaloui and Brown,
1998). Fermentation process has provided a relatively high amount of
plant nutrients particularly B, therefore after an appropriate
dilution the PFEs may be a suitable liquid fertilizer. It
has long been recognized that GAs play an important role in the
stimulation of seed germination (Chen et al. 2001), thereby the FPEs
may also induce seed germination. Other
plant nutrients particularly P, Mg, Mn and B may also be present at
appropriate concentrations to stimulate seed growth (Bellaloui and
Brown, 1998). The numbers of lactic acid bacteria (LAB) and yeasts
that were present during a wild forest noni (Morinda coreia Ham)
fermentation, the changes in its physicochemical properties and
levels of plant nutrients were investigated (Duangporn K et
al.2009)
.Higher concentratin decreases growth there may be increased
concentratin of citric acid.Results indicate that plant nutrients
present in the FPE were at an appropriate level for potential use as
a liquid fertilizer, particularly for the micronutrients such as B,
Mn and Zn.

CONCLUSION

From
this project, we can be concluded that fermented plant extract can
increase seed germination at lower concentration can use as herbal
growth promoter and at higher concentration decrease growth of
seedlings can used as herbicide. Based on these results, and because
of high chemical herbicide and growth regulator costs for farmers,
herbal growth promoter and herbicide can be replaced by them. In
addition to , they had not any environmental pollution and risks for
human being

ACKNOWLEDGEMENTS

We
are thankful to Principal Dr. S B Patil, Hutatma Rajguru
Mahavidyalaya for his timely help and support.

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