Extensive examinations
of essential part of AP2/ERF genes in growth of plant, improvement and stress
response7,12-14. For most cases, the AP2subfamily, the main elements
include organic design and organic progression, for example, the determination
of epidermal leaf cells, spikelet meristem and the design of plant organs15
as well as grain yield16,17, while the RAV subfamily showed
important functions in transduction of plant hormone, such as ethylene18,
brassinosteroids19, as well as responses to biological and abiotic
stress20,21. In addition, DREB, along with different individual in
ERF family, is mainly affected by the response to biological and abiotic stresses,
for example water scarcity22, low temperature23,24and
high salt stress25.Proline free accumulation is a common reaction to
stress in high plants26. There are several reports of positive
correlations between proline accumulation and compatibility plants with stress
conditions under drought stress and salinity27. Proline affects the
solubility of various proteins and enzymes and prevents them from changing
their nature. In plants, for example, beans and soybeans, with a decrease in
water potential, have been shown a significant increase in proline content28.

Sunflower (Helianthus
annuus L.) is one of the important components of oil seed crop and it is different
from the metabolism, physiological, and methods of regulating the etymology of
reproductive stress metabolism. This function is of unique enthusiasm for
adjusting it to high temperatures, limited access to water, high salinity and
predominant metal scrap in the soil29.Typically, the DREB subfamily
as a candidate is possible to increase the environmental tolerance of the
product. The DREB subfamily shows distinct reaction patterns according to the
ecological strategies, including low temperature (AtCBF1)23,
heat (ZmDREB2A, AtDREB1A)24,30, osmosis (CkDREB)31,
drought (OsDREB1)22,32 and lack of water and high stress (CaDREBLP1)25.
DREB provide a large number of hydration/cold genes in collaboration with the
DRE/CRT components (A/GCCGAC) available in COR/RD promoters33. Meanwhile,
a few DREB subfamily genes are reportedly positive and negative intermediary of
ABA and sugar reactions, essentially during germination and early plant
breeding stages34.

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Over-expression
of the DREB gene within the framework of plants increases salt tolerance as a
positive control35,36. Expression of OsDREB2A and OsDREB1F
enhancement increases drought stress and salinity stress in rice and Arabidopsis36.
In rice, OsDREB1A and OsDREB1F were created by cold stress. OsDREB1F
was also used for drought, salt, and ABA treatment. Over-expression of OsDREB1A
and OsDREB1F resulted in increased resistance to dry season and severe
salt susceptibility in Arabidopsis35-37. In Arabidopsis, a CBF2
mutation was distinguished by using a reverse genetic approach, in which the
CBF2/DREB1C gene was abnormal. CBF2 mutation increased resistance to drought
stress and salt. The expression analysis showed that CBF2/DREB1C was
inconsistent with the CBF1/DREB1B and CBF3/DREB1A instructions38.
Surprisingly, the DREB1/2 gene initiated under drought stress and salt,
suggesting