Purpose:

To see how movement will affect the body’s production of carbon dioxide.

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Hypothesis:

If the body experiences aerobic exercise, then carbon dioxide will be produced, making
the BTB solution in the experiment change color more rapidly.

Background:

Adenosine Triphosphate, or ATP, is a molecule produced in the Glycolysis stage of
cellular respiration that gives the body energy for all of its cellular processes. When exercising,
the body needs more energy very rapidly. The body receives this energy quickly by producing
ATP through lactic acid fermentation, a process where glucose is made into lactic acid.
However, lactic acid fermentation only produces 2 ATP molecules. To gain more energy, the
cells have to go through the Krebs Cycle and the Electron Transport Chain. These cycles
produces energy faster than Glycolysis or fermentation. After using the body’s glycogens during
the first 15 minutes of physical activity, the body starts burning lipids as energy.

Pre-Lab Questions:

What are the 3 reactions of Aerobic Cellular Respiration?
A. Glycolysis

Krebs Cycle

Electron Transport Chain

In what parts/area of the cell does cellular respiration occur?

A. Mitochondria

Materials:

?  2 small beakers

?  Bromothymol blue solution (BTB)

?  2 straws

?  Timer

?  Graduated cylinder

?  Pipette Dropper

B. Cytoplasm
3. After which reaction is CO released?

2
? Krebs Cycle

Diagram:

Procedure:

Cynthia Anderson
Katie Schluter, Paige Hawkins, Jessie Michalak, Al-Van Nguyen

1

Use the graduated cylinder to place 10mL of water into two beakers, one labeled ‘A’ and
one labeled ‘B’

Put 10 drops of Bromothymol Blue solution into each beaker

This step is timed . When a group member says ‘GO,’ begin blowing air through a straw

into Beaker A. Blow carefully and DO NOT inhale or splash!

When the solution changes color, stop blowing and record the time it took to change

color.

Now jog/exercise aerobically for 1 minute.

Repeat steps 4 and 5 using Beaker B and record the results.

Rinse the beakers and throw away the straw.

Repeat steps 1-5 with another group member and record results.

Clean workstation by rinsing out the beakers and throwing away the straw.

Observations and Data:

Beaker A (Resting)

Beaker B (Exercising)

Partner 1 Time Needed to
Change Color

7 seconds

5 seconds

Partner 2 Time Needed to
Change Color

6 seconds

4 seconds

1 Two Beakers with Colored Liquids .
pim-resources.coleparmer.com/item/l/pyrex-1000-10-brand-1000-griffin-low-form-beaker-10-ml-12-p
k-3450201.jpg.

Results and Conclusions:

Cynthia Anderson
Katie Schluter, Paige Hawkins, Jessie Michalak, Al-Van Nguyen

Through this experiment, my hypothesis was proven correct. Typically the body gets its
energy through glycolysis and lactic acid fermentation, however, during exercise large amounts
of energy are needed at a fast pace. Therefore the Krebs Cycle and Electron Transport Chain are
used to produce energy. When energy is exerted, carbon dioxide is released through the Krebs
Cycle during the process of cellular respiration. Because of this, the color of the solution
changed faster after a minute of aerobic exercise. This shows that as you exercise, you produce
more carbon dioxide and thus the color of the solution changes more rapidly.

Post-Lab Questions:

What is the equation for cellular respiration??
6 O 2+ C 6+ H 12+ O 6? 6C O 2+ 6 H 2O + 36ATP

What are the reactants for cellular respiration?

6 O 2+ C 6+ H 12+ O 6? 6C O 2

What are the products for cellular respiration?

6C O 2+ 6 H 2O + 36ATP

Which chemical process serves as the opposite reaction to cellular respiration?

Photosynthesis has the opposite reaction as cellular respiration.

List 2 types of organisms that do cellular respiration other than animals.

As well as animals, plants and bacteria undergo cellular respiration.

Other than Aerobic Cellular Respiration, what is another type of respiration used for

supplying energy?

Anaerobic Cellular Respiration is another form of respiration that can be used to supply energy.

Using your answer above, what are 2 examples of this type of respiration?

Alcoholic fermentation and lactic acid fermentation are two types of Anaerobic cellular

respiration that produce energy.

How does exercise affect the process of aerobic cellular respiration?

When exercising, the body uses its energy quickly. The body rapidly produces more energy
through aerobic cellular respiration by using the Krebs Cycle as opposed to lactic acid
fermentation.

Why is Aerobic exercise a good thing compared to anaerobic respiration/fermentation?
Aerobic Cellular Respiration is more efficient than anaerobic respiration or fermentation because

it is very quick. Aerobic respiration produces more energy and is faster than anaerobic

respiration.

What are 2 reasons why Partner 1 and Partner 2 may have had different times to change

the BTB solution yellow?

The differing amounts of glucose in the foods the test subjects ate before the experiment

could affect the outcome of the experiment.

The more glycogen present, the faster cellular respiration is.